Active Recombinant Human APOE4 protein, His-tagged

• Cat.No.: APOE4-2873H
• Product Overview: Recombinant Human APOE4 protein(Lys 19-His 317), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Loaded Biotinylated Human TREM2, His,Avitag on SA Biosensor, can bind Human APOE3, His Tag with an affinity constant of 37.1 nM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human TREM2, Fc Tag on Protein A Biosensor, can bind Human APOE3, His Tag with an affinity constant of 66.1 nM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human TREM2, Mouse IgG2a Fc Tag on Protein A Biosensor, can bind Human APOE3, His Tag with an affinity constant of 9.53 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 36.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Lys 19-His 317
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.