Active Recombinant Human ANGPT2 protein, His-tagged

• Cat.No.: ANGPT2-244H
• Product Overview: Recombinant Human ANGPT2 protein(NP_001138.1)(Lys275-Phe496), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized human ANGPT2 at 10 μg/ml (100 μl /well) can bind human Tie2-Fch, The EC50 of human Tie2-Fch is 0.1-0.3μg/ml.
• Molecular Mass: The recombinant human ANGPT2 consists of 241 amino acids and predicts a molecular mass of 27.8 kDa. As a result of glycosylation, it migrates as an approximately 31.3 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Lys275-Phe496
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123