Active Recombinant Human ANGPT2, Flag-tagged

• Cat.No.: ANGPT2-435H
• Product Overview: Recombinant Human ANGPT2(aa 19-496), fused at the N-terminus to a FLAG®-tag, was expressed in HEK 293 cells.

Specification

• Description: The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
• Source: HEK 293 cells
• Species: Human
• Tag: Flag
• Form: Lyophilized. Contains PBS with trehalose and Brij35.
• Bio-activity: Binds to the human and mouse Tie-2 receptor.
• Molecular Mass: ~70kDa (SDS-PAGE)
• Endotoxin: <0.01EU/μg purified protein (LAL test; Lonza).
• Purity: ≥95% (SDS-PAGE)
• Storage: Short Term Storage: +4°CLong Term Storage: -20°CHandling Advice: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
• Concentration: 0.1 mg /ml after reconstitution.
• Reconstitution: Reconstitute with 100μl sterile water.
• Protein length: 19-496 a.a.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; ANG2; AGPT2; Tie2-ligand; angiopoietin-2B;.angiopoietin-2a; angiopoietin-2; ANG-2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123
• Chromosome Location: 8p23.1
• Pathway: Angiopoietin receptor Tie2-mediated signaling; Cell surface interactions at the vascular wall; HIF-1 signaling pathway
• Function: metal ion binding; protein binding; receptor binding; receptor tyrosine kinase binding