Active Recombinant Human AMICA1 Protein, Fc Chimera

• Cat.No.: AMICA1-839H
• Product Overview: Recombinant Human AMICA1 (Accession # NP_694938), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Leu10
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of BT-20 human breast cancer cells.When 5 x 10^4 cells/well are added to rhAMICA/Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 55% - 75% will adhere after 1 hour at 37° C. Similar results were obtained using HeLa, A549, and T84 cell-lines. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human AMICA1, Fc Chimera has a calculated MW of 55.5 kDa (monomer). In SDS-PAGE migrates as 65-70 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile, deionized water.

Gene Information

• Gene Name: AMICA1 adhesion molecule, interacts with CXADR antigen 1 [ Homo sapiens ]
• Official Symbol: AMICA1
• Synonyms: AMICA1; adhesion molecule, interacts with CXADR antigen 1; junctional adhesion molecule-like; AMICA; Gm638; adhesion molecule AMICA; junctional adhesion molecule; dendritic cell-specific protein CREA7-1; dendritic-cell specific protein CREA7-1; dendritic-cell specific protein CREA7-4; adhesion molecule interacting with CXADR antigen 1; adhesion molecule that interacts with CXADR antigen 1; JAML; CREA7-1; CREA7-4; FLJ37080; MGC118814; MGC118815
• Gene ID: 120425
• mRNA Refseq: NM_001098526
• Protein Refseq: NP_001091996
• MIM: 609770
• UniProt ID: Q86YT9
• Chromosome Location: 11q23.3