Active Recombinant Human ACE2 Protein, His-tagged, Alexa Fluor® 488 conjugated

• Cat.No.: ACE-31H
• Product Overview: Recombinant Human Alexa Fluor® 488 ACE-2 (Gln18-Ser740) protein with a C-terminal 10-His tag was expressed in Mammalian Cells.

Specification

• Species: Human
• Source: NS0
• Tag: His
• Protein Length: 18-740 a.a.
• Description: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible.
• Form: Recombinant Human ACE2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
  Labeled with Alexa Fluor® 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Bio-activity: Measured by flow cytometry for its ability to bind Biotinylated Recombinant SARS-CoV-2 Spike RBD Fc Chimera Avi-tag conjugated streptavidin beads at 0.37-3.33 μg/mL (100 μL/well).
• Molecular Mass: 85 kDa
• N-terminal Sequence Analysis: No results obtained: Gln18 predicted
• Endotoxin: < 1.0 EU/ μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol with BSA as a carrier protein.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: ACE2 angiotensin converting enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: ACE2 angiotensin converting enzyme 2 [ Homo sapiens (human) ]
• Synonyms: ACE2; angiotensin converting enzyme 2; ACEH; angiotensin-converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin-converting enzyme-related carboxypeptidase; metalloprotease MPROT15; peptidyl-dipeptidase A; truncated angiotensin converting enzyme 2; EC 3.4.17.23
• Gene ID: 59272
• mRNA Refseq: NM_021804
• Protein Refseq: NP_068576
• MIM: 300335
• UniProt ID: Q9BYF1