Active Recombinant HIV-1 C gp120 Envelope Glycoprotein

• Cat.No.: HIV1Cgp120-102H

Specification

• Species: HIV
• Source: Insect Cells
• Tag: Non
• Description: Full length Recombinant HIV-1 C gp120 Envelope Glycoprotein produced in the Baculovirus Expression System. This protein binds to murine monoclonal antibodies of defined epitope specificity and human serum polyclonal antibodies in ELISA and Western ELISA.
• Form: Frozen Liquid
• Bio-activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp120/Anti-gp120 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent Cells.
• Purity: > 95 % by SDS-PAGE
• Applications: In-Vitro Diagnostics;
  T-Cell Activation;
  CD4 Binding.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
  ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.
• Stability: At least 2 years at -75 cemtigrade.
• Storage: Store at -75 centigrade.
• Dilutions: PBS