Active Recombinant HCMV (strain AD169) gB protein, His-tagged

• Cat.No.: gB-1492H
• Product Overview: Recombinant HCMV (strain AD169) gB protein(Ser25-Phe706), fused with His tag, was expressed in HEK293.

Specification

• Species: HCMV
• Source: HEK293
• Tag: His
• Protein Length: 25-706 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized HCMV (strain AD169) Glycoprotein B (gB), His Tag at 1 μg/mL (100 μL/well) can bind Anti-gB(HCMV)-antibody-SM5-1,Human IgG1(AM330) with a linear range of 0.1-4 ng/mL.Immobilized Anti-gB(HCMV)-antibody-SM5-1,Human IgG1(AM330) at 1 μg/mL (100 μL/well) can bind HCMV (strain AD169) Glycoprotein B (gB), His Tag with a linear range of 0.1-8 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 79.6 kDa. The protein migrates as 90-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser25-Phe706
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.