Active Recombinant H7N7 HA, His-tagged

• Cat.No.: HA-453H
• Product Overview: A DNA sequence encoding the Influenza A virus (A/equine/Kentucky/1a/ 1975(H7N7)) hemagglutinin (ACL12085.1) (Met1-Ile534) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: H7N7
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Ile534
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.4 μg/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/equine/Kentucky/ 1a/1975(H7N7)) comprises 545 amino acids and has a predicted molecular mass of 61.6 kDa. The apparent molecular mass of the protein is approximately 63.3 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.