Active Recombinant H5N3 (A/duck/Hokkaido/167/2007) HA Protein, His-tagged

• Cat.No.: HA-357V
• Product Overview: Recombinant extracellular domain (Met 1-Gln 527) of the influenza A H5N3 Hemagglutinin (A/duck/Hokkaido/167/2007) (BAG07130.2), was expressed, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: H5N3
• Tag: His
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to agglutinate human red blood cells. HA titer is 4-20 μg/mL for 1% HRBC.
• Molecular Mass: The secreted recombinant influenza A H5N3 HA (A/duck/Hokkaido/167/2007 (H5N3)) comprises 522 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-527 a.a.