Active Recombinant H5N1 HA, His-tagged

• Cat.No.: HA-437H
• Product Overview: A DNA sequence encoding the Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) hemagglutinin (AGC13463.1) (Met1-Gln531) with cleavage site mutated (RRRRK →TET, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: H5N1
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Gln531
• Description: Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of theenvelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle"s own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-8 ng/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/barnswallow/HongKong/D10-1161/2010(H5N1)) comprises 524 amino acids and has a predicted molecular mass of 59.8 kDa. The apparent molecular mass of the protein is approximately 58.3 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: HA?hemagglutinin [?Influenza A virus (A/goose/Guangdong/1/1996(H5N1))]
• Official Symbol: HA
• Synonyms: HA; hemagglutinin; HA1; HA2
• Gene ID: 3654620
• Protein Refseq: YP_308669
• UniProt ID: Q9Q0U6
• Chromosome Location: segment: segment 4