Active Recombinant H5N1 (A/bar-headed goose/Qinghai/1A/2005) HA Protein, His-tagged

• Cat.No.: HA-639V
• Product Overview: Recombinant Influenza A virus (A/bar-headed goose/Qinghai/1A/2005(H5N1)) hemagglutinin (ABF93441.1) (Met1-Gln527), termed as HA, was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: H5N1
• Tag: His
• Predicted N Terminal: Asp 17
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC.
• Molecular Mass: The recombinant HA subunit of iInfluenza A virus (A/bar-headed goose/Qinghai/1A/2005(H5N1) consists 522 amino acids and predicts a molecular mass of 59.6 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-527 a.a.