Active Recombinant H3N2 (A/Sydney/5/1997) HA Protein, His-tagged

• Cat.No.: HA-669V
• Product Overview: Recombinant Influenza A virus (A/Sydney/5/1997 (H3N2)) hemagglutinin (ACO95259.1) (Met1-Trp530), termed as HA, was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: H3N2
• Source: Insect Cells
• Tag: His
• Protein Length: 1-530 a.a.
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.03-0.15μg/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/Sydney/5/1997 (H3N2)) consists 525 amino acids and predicts a molecular mass of 59.3 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.