Active Recombinant H1N1 (A/USSR/90/77) HA Protein, His-tagged

• Cat.No.: HA-656V
• Product Overview: Recombinant Influenza A virus (A/USSR/90/77(H1N1)) hemagglutinin (P03453.2) (Met1-Gln529) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: His
• Protein Length: 1-529 a.a.
• Predicted N Terminal: Asp 18
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.5, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.01-0.04 μg/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/USSR/90/1977(H1N1))comprises 523 amino acids and has a predicted molecular mass of 59.4 kDa. The apparent molecular mass of the protein is approximately 65.3 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.