Active Recombinant H10N3 HA, His-tagged

• Cat.No.: HA-411H
• Product Overview: A DNA sequence encoding the Influenza A virus (A/mallard/Minnesota/Sg-00194/2007(H10N3)) hemagglutinin (Met1-Val525) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: H10N3
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Val525
• Form: Lyophilized from sterile PBS, pH7.4.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.2. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 2-8 ng/mL for 1% GRBC.
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/mallard/Minnesota/Sg-00194/2007(H10N3)) comprises 520 amino acids and has a predicted molecular mass of 57.9 kDa. The apparent molecular mass of the protein is approximately 64 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.