Active Recombinant Full Length Human ubiquitin specific peptidase 5 Protein, His Tagged

• Cat.No.: USP5-12HFL
• Product Overview: Recombinant full length human USP5 was expressed in E. coli cells using an N-terminal His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-858aa
• Description: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored ''Lys-48''-linked polyubiquitin disassembly. It binds linear and ''Lys-63''-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
• Tag: N-His
• Molecular Mass: 98 kDa
• Applications: WB,Activity Assay
• Stability: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300mM NaCl, 50-200mM imidazole, 0-0.1mM PMSF, 0.2-1.0mM DTT, 10-25% glycerol
• Shipping: Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
• References: 1. Reyes-Turcu F.E., et al., 2006, Cell 124:1197-1208.
  2. Reyes-Turcu F.E., et al., 2008, J. Biol. Chem. 283:19581-19592.
  3. Dayal S., et al., 2009, J. Biol. Chem. 284:5030-5041.
  4. Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.

Gene Information

• Gene Name: USP5 ubiquitin specific peptidase 5 [ Homo sapiens (human) ]
• Official Symbol: USP5
• Synonyms: USP5; ubiquitin specific peptidase 5 (isopeptidase T); ubiquitin specific protease 5 (isopeptidase T); ubiquitin carboxyl-terminal hydrolase 5; IsoT; isopeptidase T; ubiquitin isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin-specific-processing protease 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ISOT
• Gene ID: 8078
• mRNA Refseq: NM_001098536
• Protein Refseq: NP_001092006
• MIM: 601447
• UniProt ID: P45974