Active Recombinant Full Length Human SERPINC1, His-tagged

• Cat.No.: SERPINC1-7274H
• Product Overview: A DNA sequence encoding the Human SerpinC1 (Met 1-Lys 464) was expressed in Human Cells, with a C-terminal His tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Lys 464
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.
  The IC50 value is < 5 nM.
• Molecular Mass: The recombinant human SerpinC1 consists of 443 amino acids and has a predicted molecular mass of 50.5 kDa. In SDS-PAGE, the apparent molecular mass of rhSerpinC1 is approximately 55-60 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SERPINC1 serpin peptidase inhibitor, clade C (antithrombin), member 1 [ Homo sapiens ]
• Official Symbol: SERPINC1
• Synonyms: SERPINC1; serpin peptidase inhibitor, clade C (antithrombin), member 1; AT3, serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; antithrombin-III; antithrombin (aa 375 432); antithrombin III; ATIII; coding sequence signal peptide antithrombin part 1; MGC22579; signal peptide antithrombin part 1; serpin C1; serine-cysteine proteinase inhibitor clade C member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; AT3; AT3D; THPH7
• Gene ID: 462
• mRNA Refseq: NM_000488
• Protein Refseq: NP_000479
• MIM: 107300
• UniProt ID: P01008