Active Recombinant Full Length Human PRKCI Protein, GST-tagged

• Cat.No.: PRKCI-8859HF
• Product Overview: Full-length human PKCι[1-587(end) amino acids of accession number NP_002731.3] was expressed as N- terminal GST-fusion protein (94 kDa) using baculovirus expression system. GST-PKCι was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Protein length: 1-587
• Purity: 97%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase C, iota
• Official Symbol: PRKCI
• Synonyms: PRKCI;DXS1179E;PKCI;nPKC-iota
• Protein Refseq: NP_002731.3
• UniProt ID: P41743