Active Recombinant Full Length Human PRKCH Protein, DDK-tagged, Biotinylated

• Cat.No.: PRKCH-1440HF
• Product Overview: Full-length human PKCη [1-683(end) amino acids of accession number NP_006246] was expressed as N- terminal DYKDDDDK tagged, biotinylated protein (82 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: Flag
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Lipid Activator. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
• Protein length: 1-683(end)
• Purity: 97%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: protein kinase C, eta
• Official Symbol: PRKCH
• Synonyms: PRKCH;PKC-L;PKCL;PRKCL;nPKC-eta
• Protein Refseq: NP_006246
• UniProt ID: P24723