Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged
Cat.No. : | MAP2K1-5481HF |
Product Overview : | Full-length human MAP2K1 [1-393(end) amino acids and K97R of accession number NP_002746] was expressed as N-terminal GST-fusion protein (71 kDa) using baculovirus expression system. GST- MAP2K1 was purified by using glutathione sepharose chromatography. |
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Species : | Human |
Source : | Sf21 Cells |
Tag : | GST |
Protein Length : | 1-393(end) |
Form : | 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5 |
Bio-activity : | The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG) . |
Purity : | 100% |
Applications : | SDS-PAGE |
Storage : | Store at -80C. Avoid repeating freeze-thaws. |
Gene Name | mitogen-activated protein kinase kinase 1 |
Official Symbol | MAP2K1 |
Synonyms | MAP2K1;CFC3;MAPKK1;MEK1;MKK1;PRKMK1 |
Protein Refseq | NP_002746.1 |
UniProt ID | Q02750 |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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