Active Recombinant Full Length human AKR7A3 protein, Tag Free

• Cat.No.: AKR7A3-26150TH
• Product Overview: Active Recombinant Full Length human AKR7A3 protein,Tag Free,expressed in E.coli.

Specification

• Description: Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3 was expressed in E. coli and purified by using conventional chromatography techniques.
• Source: E. coli
• Species: Human
• Tag: Non
• Protein length: 1-331aa
• Bio-activity: Specific activity is > 800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.
• Molecular Mass: 37.7 kDa
• AA Sequence: MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS EGQSETILGG LGLRLGGSDC RVKIDTKAIP
  LFGNSLKPDS LRFQLETSLK RLQCPRVDLF YLHMPDHSTP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV AEICTLCKSN
  GWILPTVYQG MYNAITRQVE TELFPCLRHF GLRFYAFNPL AGGLLTGKYK YEDKDGKQPV GRFFGNTWAE MYRNRYWKEH
  HFEGIALVEK ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA AAEEGPLEPA VVDAFNQAWH
  LVAHECPNYF R
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)
• Storage Buffer: Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
• GeneID: 22977
• References: Ahmed MM., et al. (2011) Hepatology. 54(4):1322-32.
  Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21(5):1134-42.

Gene Information

• Gene Name: AKR7A3 aldo-keto reductase family 7 member A3 [ Homo sapiens (human) ]
• Official Symbol: AKR7A3
• Synonyms: Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2(AFAR2), AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2,AKR7A3
• mRNA Refseq: NM_012067
• Protein Refseq: NP_036199
• MIM: 608477
• UniProt ID: O95154