Active Recombinant Equine Interleukin 15

• Cat.No.: IL15-253E
• Product Overview: Recombinant Equine IL-15 was expressed inYeast. MW= 13.0 kDa(114 aa).

Specification

• Species: Equine
• Source: Yeast
• Tag: Non
• Description: Interleukin 15 (IL-15) is a novel cytokine that uses beta- and gamma-chain of IL-2R for signal transduction and is structurally similarity to IL-2. IL-15 is secreted by activated monocytes/macrophages following infection by virus. This cytokine induces cell proliferation of natural killer cells. This cytokine and interleukin 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other"s activity. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. IL-15 can recruit and activate T lymphocytes in the synovial membrane, thereby contributing to Rheumatoid arthritis pathogenesis.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: NWQDVISDLK RIEDLIQSIH VDATLYTESD AHPNCKVTAM KCFLLELHVI SHESRNEDIK ETVENLIILA NSSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INPS.
• Bioactivity: The biological activity of recombinant equine IL-15 measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is typically 50 - 75 ng/mL.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL15 interleukin 15 [ Equus caballus ]
• Synonyms: interleukin 15; IL15
• Gene ID: 100034058
• mRNA Refseq: NM_001163986.1
• Protein Refseq: NP_001157458.1
• Chromosome Location: 2
• Pathway: Cytokine-cytokine receptor interaction; Intestinal immune network for IgA production; Jak-STAT signaling pathway