Active Recombinant Equine IL4

• Cat.No.: IL4-256E
• Product Overview: Equine IL-4 was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.

Specification

• Species: Equine
• Source: Yeast
• Tag: Non
• Description: Interleukin-4 (IL-4) induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B-cell class switching to IgE, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.
• Form: Lyophilized without carrier protein.
• Bio-activity: The biological activity of recombinant equine IL-4 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 40-60 ng/mL.
• Molecular Mass: Equine IL-4 has a predicted molecular weight of 12.8 kDa.
• AA Sequence: GCKYDITLQE IIKTLNNLTD GKGKNSCMEL TVADAFAGPK NTDGKEICRA AKVLQQLYKR HDRSLIKECL SGLDRNLKGM ANGTCCTVNE AKKSTLKDFL ERLKTIMKEK YSKC (114)
• Purity: >95% as visualized by SDS-PAGE analysis.
• Applications: The Equine IL-4 protein can be used in cell culture, as an IL-4 ELISA Standard, and as a Western Blot Control.
• Storage: Stable for up to twelve months from date of receipt at -20oC. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.
• Reconstitution: Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.

Gene Information

• Gene Name: IL4 interleukin 4 [ Equus caballus (horse) ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; IL-4; BSF-1; interleukin-4; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1
• Gene ID: 100034225
• mRNA Refseq: NM_001082519
• Protein Refseq: NP_001075988
• UniProt ID: P42202
• Chromosome Location: 14
• Function: Allograft rejection; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction