Active Recombinant Cynomolgus/Rhesus TNFRSF1B protein, hFc-tagged

• Cat.No.: TNFRSF1B-2184C
• Product Overview: Recombinant Cynomolgus/Rhesus TNFRSF1B protein(XP_005544817.1 & NP_001253134.1)(Met1-Asp257), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Asp257
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 7-32 ng/mL in the presence of 1 ng/mL of cynoTNFα.
• Molecular Mass: The recombinant cynomolgus / rhesus TNFRSF1B is a disulfide-linked homodimer. The reduced monomer comprises 476 amino acids and has a calculated molecular mass of 52.1 KDa.The apparent molecular mass of the protein is approximately 68 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.