Active Recombinant Cynomolgus/Rhesus PVRIG Protein, His-tagged

• Cat.No.: PVRIG-235C
• Product Overview: Recombinant Cynomolgus/Rhesus PVRIG Protein (XP_005549281.1/XP_014989941.2) (Thr41-Leu172) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2hFc at 2 μg/ml (100 μl/well) can bind Cynomolgus PVRIG(41T-172L) His, the EC50 of Cynomolgus PVRIG(41T-172L) His is 150-450 ng/mL.
• Molecular Mass: The recombinant cynomolgus / rhesus PVRIG consists of 143 amino acids and predicts a molecular mass of 15.3 kDa. As a result of glycosylation, It migrates as an approximately 25-30 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Thr41-Leu172
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.