Active Recombinant Cynomolgus/Rhesus macaque TNFRSF17 protein, His-Avi-tagged, Biotinylated

• Cat.No.: TNFRSF17-2923C
• Product Overview: Biotinylated Recombinant Cynomolgus/Rhesus macaque TNFRSF17 protein(Met 1-Ala 53), fused with His Avi tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Avi&His
• Protein Length: Met 1-Ala 53
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human APRIL, His,Flag Tag, active trimer at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag with a linear range of 0.2-1 ng/mL.Immobilized Biotinylated Cynomolgus / Rhesus macaque BCMA, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human BAFF, Fc Tag, active trimer with a linear range of 0.2-5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 9.6 kDa. The protein migrates as 15-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.