Active Recombinant Cynomolgus / Rhesus macaque IL15 protein, His-tagged

• Cat.No.: IL15-0177C
• Product Overview: Recombinant Cynomolgus / Rhesus macaque IL15 protein(Asn49-Ser162), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: 49-162 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-15 R alpha, Fc Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-15, His Tag with a linear range of 1-8 ng/mL.Immobilized Anti-IL-15 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque IL-15, His Tag with a linear range of 2-16 ng/mL.Immobilized Cynomolgus / Rhesus macaque IL-15, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (MALS verified) with a linear range of 0.039-0.625 μg/mL.Immobilized Cynomolgus / Rhesus macaque IL-15, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag with a linear range of 0.078-1.25 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 14.9 kDa. The protein migrates as 14 kDa and 16-18 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asn49-Ser162
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: IL15