Active Recombinant Cynomolgus/Rhesus macaque CD244 protein, His-tagged

• Cat.No.: CD244-2858C
• Product Overview: Recombinant Cynomolgus/Rhesus macaque CD244 protein(Leu 24-Gln 186), fused with His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: His
• ProteinLength: Leu 24-Gln 186
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus / Rhesus macaque 4-1BB, His Tag at 1 μg/mL (100 μL/well) can bind Human 4‑1BB Ligand, Fc Tag with a linear range of 0.05-0.5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 19.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.