Active Recombinant Cynomolgus/Rhesus CTLA4 protein, His-tagged

• Cat.No.: CTLA4-354C
• Product Overview: Recombinant Cynomolgus/Rhesus CTLA4 protein(XP_005574071.1/Q9BDC4) (Met1-Asp161) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized cynomolgus/rhesus CTLA4-His at 10 μg/ml (100 μl/well) can bind Cynomolgus CD80-Fc, The EC50 of cynomolgus/rhesus CD80-Fc is 0.02-0.04 μg/ml.
• Molecular Mass: The recombinant cynomolgus / rhesus CTLA4 comprises 136 amino acids and has a calculated molecular mass of 14.9 KDa. The apparent molecular mass of it is approximately 24 and 21 KDa respectively in SDS-PAGE due to different glycosylation.
• Protein length: Met1-Asp161
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95% as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte associated protein 4 [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CTLA4
• Synonyms: CTLA4
• Gene ID: 102115124
• mRNA Refseq: XM_005574014
• Protein Refseq: XP_005574071