Active Recombinant Cynomolgus NRP1 protein(Met1-Gly613), His-tagged

• Cat.No.: NRP1-1218C
• Product Overview: Recombinant Cynomolgus NRP1 (XP_005564993.1) (Met1-Gly613) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• ProteinLength: Met1-Gly613
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized VEGF165 Protein, Human, Cynomolgus, Recombinant at 2 μg/mL (100 μL/well) can bind Neuropilin-1 Protein, Cynomolgus, Recombinant (His Tag), the EC50 is 260-980 ng/mL.
• Molecular Mass: The recombinant cynomolgus NRP1 consists 603 amino acids and predicts a molecular mass of 68.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.