Active Recombinant Cynomolgus MET Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: MET-184CAF647
• Product Overview: A DNA sequence encoding the cynomolgus/rhesus MET (NP_001162100.1) (Met1-Thr932) (Alexa Fluor 647 conjugated) was expressed with a polyhistidine tag at the C-terminus. Cynomolgus and Rhesus MET sequences are identical.

Specification

• Source: HEK293
• Species: Monkey
• Tag: His
• Form: Lyophilized
• Bio-activity: Immobilized Cynomolgus MET-His at 10 μg/mL (100 μL/well) can bind biotinylated Cynomolgus HGF, The EC50 of biotinylated Cynomolgus HGF is 0.11-0.27 μg/mL.
• Molecular Mass: The recombinant cynomolgus/rhesus MET comprises 919 amino acids and has a calculated molecular mass of 103.1 kDa. The apparent molecular mass of it is approximately 128.2, 77.6 and 45.7 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Thr932， 919
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: (4.6+58.2+34.7) % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: METmet proto-oncogene (hepatocyte growth factor receptor) [Macaca mulatta(Rhesus monkey) ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; NP_001162100.1; EC 2.7.10.1
• Gene ID: 704562
• mRNA Refseq: NM_001168629
• Protein Refseq: NP_001162100