Active Recombinant Cynomolgus LGR5 protein, His-tagged

• Cat.No.: LGR5-0348C
• Product Overview: Recombinant Cynomolgus LGR5 protein(Ser22-Gly557), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus LGR5, His Tag at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag with a linear range of 0.2-5 ng/mL.Immobilized Human R-Spondin 1 (21-146), Fc Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus LGR5, His Tag with a linear range of 0.1-10 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 61.7 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser22-Gly557
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 22-557 a.a.

Gene Information

• Official Symbol: LGR5