Active Recombinant Cynomolgus IGF1R Protein, His-tagged

• Cat.No.: IGF1R-2216C
• Product Overview: Recombinant Cynomolgus IGF1R Protein(G7P9I7-1)(Glu 31 - Asn 932), fused with His tag, was expressed in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 5-78 ng/mL.
• Molecular Mass: This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.1 kDa and 23.8 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Glu 31 - Asn 932
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.