Active Recombinant Cynomolgus IFNA13 protein, hFc-tagged

• Cat.No.: IFNA13-885C
• Product Overview: Recombinant Cynomolgus IFNA13 protein (G7NFW4) (Met1-Glu190) was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV).
  The ED50 for this effect is typically 0.5-3 ng/mL.
• Molecular Mass: The recombinant cynomolgus IFNA13 is a disulfide-linked homodimer. The reduced monomer comprises 407 amino acids and has a calculated molecular mass of 46.4 KDa.The apparent molecular mass of the protein is approximately 47 KDa respectively in SDS-PAGE.
• Protein length: Met1-Glu190
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.