Active Recombinant Cynomolgus EPO protein(Met1-Arg192), His-tagged

• Cat.No.: EPO-1196C
• Product Overview: Recombinant Cynomolgus Epo (XP_005549312.1) (Met1-Arg192) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg192
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-5 ng/mL.
• Molecular Mass: The recombinant cynomolgus Epo consists 176 amino acids and predicts a molecular mass of 19.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.