Active Recombinant Cynomolgus CYP2C9 Protein

Description :	CYP2C9 mediated oxidation of valproate, which is the minor metabolic pathway in adults, appears to become the principal route in children. Genetic and non-genetic variations in CYP2C9 activity can result in significant inter- and intra-individual differences in valproate pharmacokinetics and valproate induced adverse reactions.
Source :	E. coli
Species :	Cynomolgus
Bio-activity :	Cytochrome c Reductase Activity: 191 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: Testosterone 17-oxidase Linear with time up to: 30 min(Determined at 15 µM substrate, using a P450 concentration of 10 pmol/ml) Linear with P450 up to: 50 pmol/ml(Determined at 15 µM substrate, using an incubation time of 5 min) Vmax: 15 pmol/min/pmol CYP Km: 15 μM
Usage :	For laboratory (research) purposes only
Storage :	Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration :	P450 Concentration: 2.8 nmol/ml Protein Concentration: 9.4 mg/ml Specific P450 Content: 298 pmol/mg protein
Storage Buffer :	50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Name	CYP2C9 cytochrome P450 family 2 subfamily C member 9 [ Macaca fascicularis (crab-eating macaque) ]
Official Symbol	CYP2C9
Synonyms	CYP2C9; cytochrome P450 family 2 subfamily C member 9; CYP2C43; cytochrome P450 2C9-like; cytochrome P450 family 2 subfamily C member 9; cytochrome P450 2C43; cytochrome P450, family 2, subfamily C, polypeptide 9
Gene ID	102115450
mRNA Refseq	NM_001287625
Protein Refseq	NP_001274554
UniProt ID	Q0HA18