Active Recombinant Cynomolgus CSF1R protein, Fc-tagged

• Cat.No.: CSF1R-0510C
• Product Overview: Recombinant Cynomolgus CSF1R protein(Ile99-Pro596), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: 99-596 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human M-CSF, His,Avitag at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag with a linear range of 1-39 ng/mL. Immobilized Human M-CSF, His Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus M-CSF R, Fc Tag with a linear range of 1-39 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 81.9 kDa. As a result of glycosylation, the protein migrates as 110-125 kDa under reducing (R) condition, and 200- 240 kDa under non-reducing (NR) condition (SDS-PAGE).
• Proteinlength: Ile99-Pro596
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.