Active Recombinant COVID-19 Spike S1 (D614G) protein, His-tagged

• Cat.No.: Spike-219V
• Product Overview: Recombinant COVID-19 Spike S1 (D614G) protein (Val16-Arg685) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293).

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind 2019-nCoV S1(D614G)h, the EC50 of 2019-nCoV S1(D614G) is 250-650 ng/mL.
  2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein with an affinity constant of 57.7 nM as determined by Octet RED System.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein consists of 681 amino acids and predicts a molecular mass of 76.41 kDa.
• Protein length: Val16-Arg685(D614G)
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.