Active Recombinant COVID-19 Spike RBD protein, mFc-tagged

• Cat.No.: Spike-4729V
• Product Overview: Recombinant COVID-19 protein(Arg 319 - Phe 541), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: mFc
• Form: Supplied as 0.2 μm filtered solution in 10 mM PB, Arginine, pH7.4.
• Bio-activity: Immobilized SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 with a linear range of 0.2-3 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 52.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Arg 319 - Phe 541
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Please avoid repeated freeze-thaw cycles. This product is stable after at:
  The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2