Active Recombinant COVID-19 Spike protein S1 subunit, hFc-tagged

• Cat.No.: Spike-192V
• Product Overview: Recombinant COVID-19 Spike protein S1 subunit was fused to Human IgG1 Fc tag at C-terminus and expressed in human cells.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) is 10-50 ng/mL.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) consists of 908 amino acids and predicts a molecular mass of 101.7 kDa.
• Protein length: Val16-Arg685
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.