Active Recombinant Canine PDCD1 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: PDCD1-1057CAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant canine PDCD1 (XP_543338.3) (Met1-Leu169) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Dog
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized canine PDCD1-Fc at 10 μg/mL (100 μL/well) can bind biotinylated canine PDCD1-Fc, The EC50 of biotinylated canine PDCD1-Fc is 0.16-0.4 μg/mL.
• Molecular Mass: 43 kDa
• Protein length: 383
• N-terminal Sequence Analysis: Leu 25
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > (20.2+76.0) % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Canis lupus familiaris ]
• Official Symbol: PDCD1
• Synonyms: PD-1; programmed death 1
• Gene ID: 486213
• mRNA Refseq: NM_001314097
• Protein Refseq: NP_001301026