Active Recombinant Canine MET protein, Fc-tagged

• Cat.No.: MET-1556C
• Product Overview: Recombinant Canine MET protein(Glu25-Leu935), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Canine
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Canine HGF R,Fc Tag at 5 μg/mL (100 μL/well) can bind Human HGF, His Tag with a linear range of 2-16 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 128.4 kDa. The protein migrates as 35-40 kDa and 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu25-Leu935
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 25-935 a.a.