Active Recombinant Canine IL1R2 protein, hFc-tagged

• Cat.No.: IL1R2-2178C
• Product Overview: Recombinant Canine IL1R2 protein(E2RKW1)(Met1-Glu343), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Glu343
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Canine IL1R2-Fc3 at 10 μg/ml (100 μl/well) can bind biotinylated human IL1B-His, The EC50 of biotinylated human IL1B-His is 0.08-0.2 μg/ml.
• Molecular Mass: The recombinant canine IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 571 amino acids and has a predicted molecular mass of 64.8 kDa. The apparent molecular mass of the protein is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.