Active Recombinant Canine IL13RA2 protein, His-tagged

• Cat.No.: IL13RA2-258C
• Product Overview: Recombinant Canine IL13RA2 protein(Q95LF0)(Met1-Thr338), fused with His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr338
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.01-0.04 μg/mL.
• Molecular Mass: The recombinant canine IL13RA2 comprises 328 amino acids and has a predicted molecular mass of 38.6 kDa. The apparent molecular mass of the protein is approximately 39-46 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.