Active Recombinant Bovine TNF Protein

• Cat.No.: TNF-716B
• Product Overview: Recombinant Bovine TNF Protein is produced by E.coli expression system.

Specification

• Species: Bovine
• Source: E.coli
• Tag: Non
• Protein Length: 78-234 aa
• Description: TNF-alpha, also known as tumor necrosis factor, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Bovine TNF-alpha was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In Phosphate buffer saline(pH 7.4) containing 10 % glycerol.
• Bio-activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 15 ng/ml.
• Molecular Mass: 17.5 kDa
• AA Sequence: MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL
• Endotoxin: < 1.0 EU per 1 ug of protein (determined by LAL method)
• Purity: > 90 % by SDS - PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TNF tumor necrosis factor [ Bos taurus (cattle) ]
• Official Symbol: TNF
• Synonyms: Tumor necrosis factor; TNF-a; TNF; TNFa
• Gene ID: 280943
• mRNA Refseq: NM_019397
• Protein Refseq: NP_776391
• UniProt ID: Q06599