Active Native Wheat Triticum vulgaris Lectin (WGA), HRP-Conjugated

• Cat.No.: WGA-384W
• Product Overview: Native Wheat Triticum vulgaris Lectin (WGA) - HRP (Horseradish Peroxidase)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: N-Acetylglucosamine
  Inhibiting or Eluting Sugar: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
  Divalent Ions: Ca++
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Carbohydrate Specificity: N-Acetylglucosamine

Specification

• Description: Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells. Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Triticum vulgaris Lectin (WGA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.
• Source: Wheat
• Species: Wheat
• Form: Liquid
• Bio-activity: 10 μg/ml will agglutinate human erythrocytes nonspecifically.
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: Store frozen at -20 centigrade in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 centigrade for short term use. Clarify by centrifugation, if needed.
• Concentration: 2 mg/mL

Gene Information

• Official Symbol: WGA
• Synonyms: Triticum vulgaris Lectin; WGA