Active Native Wheat Triticum vulgaris Lectin (WGA), FITC-Conjugated

• Cat.No.: WGA-334W
• Product Overview: Native Wheat Triticum vulgaris Lectin (WGA) - FITC (Fluorescein)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: N-Acetylglucosamine
  Inhibiting or Eluting Sugar: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
  Divalent Ions: Ca++
  Excitation: 495 nm
  Emission: 515 nm
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Fluorescence: Green
  Carbohydrate Specificity: N-Acetylglucosamine

Specification

• Description: Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells. Affinity purified WGA is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.
• Source: Wheat
• Species: Wheat
• Form: Liquid
• Bio-activity: 10 μg/ml will agglutinate human erythrocytes nonspecifically.
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: Store frozen at -20 centigrade in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 centigrade for short term use. Clarify by centrifugation, if needed.
• Concentration: 2 mg/mL

Gene Information

• Official Symbol: WGA
• Synonyms: Triticum vulgaris Lectin; WGA