Active Native Wheat Triticum vulgaris Lectin (WGA), Biotinylated-Conjugated

• Cat.No.: WGA-089W
• Product Overview: Native Wheat Triticum vulgaris Lectin (WGA) - Biotinylated
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: N-Acetylglucosamine
  Inhibiting or Eluting Sugar: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
  Divalent Ions: Ca++
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Carbohydrate Specificity: N-Acetylglucosamine

Specification

• Description: Wheat germ agglutinin (WGA) is a lectin that is affinity purified from Triticum vulgaris and non-enzymatically binds to N-acetyl-D-glucosamine and sialic acid residues of glycoproteins and glycolipids. This lectin protects Triticum vulgaris from insects, yeast and bacteria. WGA consists of two subunits and has a molecular weight of 36 kDa. It is an acidic protein and has mitogenic activity toward lymphocytes. It agglutinates erythrocytes and most types of malignant cells. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated WGA has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated WGA allows for more sensitive detection in ELISA and Western-blotting applications.
• Source: Wheat
• Species: Wheat
• Form: Liquid
• Bio-activity: Less than 4 μg/ml will agglutinate human type O erythrocytes. Less than 1 mg/ml will agglutinate neuraminidase-treated erythrocytes.
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: Store frozen at -20 centigrade in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 centigrade for short term use.
• Concentration: 2 mg/mL

Gene Information

• Official Symbol: WGA
• Synonyms: Triticum vulgaris Lectin; WGA