Active Native Sambucus Nigra Lectin Protein, Agarose bound
Cat.No. : | Lectin-1837S |
Product Overview : | This product is the Agarose bound Sambucus Nigra Lectin and has sugar specificity against Sialic Acid. |
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Species : | Sambucus nigra |
Source : | Sambucus nigra |
Tag : | Non |
Description : | Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. SNA has been reported to inhibit cell-free protein synthesis. |
Form : | 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, 20 mM lactose |
Bio-activity : | Carbohydrate-Binding Specificity: Sialic Acid |
Molecular Mass : | 140 kDa |
Applications : | Glycobiology, Affinity Chromatography |
Usage : | 1, Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. 2, Suggested conditions for eluting glycoconjugates: 0.5 M lactose in buffered saline followed by 0.5 M lactose in 0.2 M acetic acid. |
Storage : | Refrigerate. DO NOT FREEZE. |
Concentration : | 3.0 mg/ml of settled gel |
Synonyms | Lectin; SNA; EBL |
UniProt ID | O04366 |
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Not For Human Consumption!
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