Active Native Mouse Fibrinogen

• Cat.No.: Fga-299M

Specification

• Species: Mouse
• Source: Plasma
• Tag: Non
• Description: The thrombin (IIa) catalyzed cleavage of soluble fibrinogen (Fbg) to form fibrin (Fbn) is the terminal proteolytic event in the coagulation cascade. These soluble Fbn monomers spontaneously polymerize to form an insoluble Fbn network which is stabilized by the factor XIIIa catalyzed crosslinking of lys and glu residues of α and γ chains. This Fbn network is the major protein component of the hemostatic plug. Plasma fibrinogen is large glycoprotein (Mr=340,000) synthesized in the liver and circulating at a concentration of 2.6 mg/ml. It is a disulfide linked dimer composed of 3 pairs of disulfide linked non-identical polypeptide chains (Aα, Bβ and γ). Notable features of the Aα chain are the N-terminal peptide (fibrinopeptide A (FPA, 1-16)), factor XIIIa crosslinking sites and 2 phosphorylation sites. When synthesized, Fbg is fully phosphorylated, but circulates at only 20-30% phosphorylation. The Bβ chain contains fibrinopeptide B (FPB, 1-14), one of the 3 N-linked carbohydrate moieties (Mr=2500) and an N-terminal pyroglutamic acid. The γ chain contains the other N-linked glycosylation site and a factor XIIIa crosslinking sites. The 2 elongated subunits ((AαBβγ)2) are aligned in an antiparallel manner forming a trinodular arrangement of the six chains. The nodes are formed by disulfide rings between the 3 parallel chains. The central node (n-disulfide knot, E domain) is formed by the N-termini of all six chains held together by 11 disulfide bonds. This region contains the 2 IIa-sensitive sites. The release of FPA by cleavage at R16-G17 generates Fbn I, exposing a polymerization site on the Aα chain. These regions bind to complimentary regions on the D domain of Fbn to form protofibrils. Subsequent IIa cleavage of FPB (R14-G15) from the Bβ chain exposes additional polymerization sites and promotes lateral growth of the Fbn network.
• Form: 20 mM NaCit, 20 mM NaPO4, pH 7.3
• Bio-activity: Thrombin inhibition
• Purity: >95% by SDS-PAGE. NOT tissue/cell culture grade. Not tested for endotoxin.
• Characteristic: Extinction coefficient: 15.1, Plasma concentration: 2.6 mg/ml, Isoelectric point: 5.1-6.3, Percent carbohydrate:0.03,
• Storage: 4°C