Active Native Bovine alpha-Thrombin-BFPRck, Biotin-tagged

• Cat.No.: Thrombin-30B
• Product Overview: Native Bovine alpha-Thrombin was purified from Plasma. FPRck is Phe-Pro-Arg chloromethylketone (CMK) and BFPRck is biotinylated FPRck.

Specification

• Species: Bovine
• Source: Plasma
• Tag: Non
• Description: Alpha-thrombin is a highly specific serine protease generated by proteolytic activation of the zymogen prothrombin. During coagulation, thrombin cleaves fibrinogen to form fibrin, leading to the ultimate step in coagulation, the formation of a fibrin clot. Thrombin is also responsible for feedback activation of the procofactors factor V and factor VIII. Thrombin has also been reported to activate factor XIII and platelets, and also functions as a vasoconstrictor protein. The procoagulant activity of thrombin is arrested in two ways: 1) inhibition by either heparin cofactor II or the antithrombin III/heparin complex; or 2) complex formation with thrombomodulin. Formation of the thrombin/thrombomodulin complex results in the inability of thrombin to cleave fibrinogen and activate factors V and VIII, but increases the efficiency of thrombin for activation of the anticoagulant, protein C.
• Form: 20 mM Hepes, 150 mM NaCl, pH 7.4
• Bio-activity: approximately 3800 NIH units/mg
• Molecular Mass: 36700 Da
• Purity: >95% by SDS-PAGE
• Storage: Glyercol formulation: -20°CHepes formulation: -80°C